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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in many tumors, which leads to chromosome instability, and thus might lead to tumorigenesis. This protein was identified to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a significant part in advertising and marketing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually explained. Two extra splice variants have been claimed but thorough nucleotide sequence data is not really however offered.
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Blocking peptides are peptides that bind especially for the focus on antibody and block antibody binding. These peptide ordinarily consists of the epitope identified by the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to guarantee the best possible benefits are...
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This manual outlines more attainable optimization of mobile and immunoassay parameters to be sure the absolute best effects are...
Antibodies certain to the blocking peptide not bind to your epitope over the concentrate on protein. By evaluating the staining in the blocked antibody versus the antibody on your own, you can see which staining is precise.
Concentrate on Details Cyclin E belongs on the extremely conserved cyclin relatives, whose members are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins show distinctive expression and degradation designs which contribute to your temporal coordination of every mitotic occasion. Cyclin E forms a fancy with and functions as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and is also degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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